Lab 27 is a sealed and self-sustaining ecosphere located a half mile below Global Dynamics. It covers 12,000 acres and includes a 800 sq. meter lake, savanna grasslands, and a rainforest (where the human habitat is located). The plant and animal life of Lab 27 are all real, but weather and the day/night cycle are simulated.

Inhabiting Lab 27 at the time of Season 3 was a 10-man research team (What About Bob? ) who had been living there for 11 years.

Douglas Fargo was known to hack into the video feeds from Lab 27 for entertainment purposes. He and Vincent were viewing buddies for this uniquely Eurekan reality program.